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  • 顔出したが また沈んでいきま~すwww

  • ここは ATMを設置している 
    コンビニ スーパー 商業施設 ショッピングモール 駅 空港
    ATMを 撤去しない限り(海外進出による のれん代などの 特別損失がない限り)
    永遠に最終利益200億円~300億円が計上される
    他業種と違い ほぼ景気に左右されません
    赤字なんて ありえませんよ

  • 銀行が店舗減らして、ATMも大幅に削減する今後
    市場を独占できる可能性がある 
    いわゆる 残存者利益というやつです

  • 配当金の入金予定の
    お知らせのメール
    いただきました

  • 2020年3月期 業績予想の開示 待つだけで良し
    年後半には 8000~10000

  • 株主に還元なんて一切しなくていい
    ガンガン先行投資してくれ
    IRは早めにしてくれよ

  • 75日移動平均線
    ぶち抜いてきました(^_^)

  • 出来高をともなっての4連騰
    これは本物だな

  • 268円で
    10万株買っててよかった

  • 割安か
    割高か
    株価は市場が決めるんだ

  • ネット広告・EC、合併・買収に集中すれば良かったのに

  • 陽線五本
    いってほしい

  • いっても
    実質無借金
    税引後利益
    数百億~1000億円の優良企業だからな

  • 2019/02/03 22:41

    To confirm that S-nitrosylation without NO generation can rescue β-AR from desensitization and to obtain practically useful drugs, we have developed a series of water-soluble compounds that may induce S-nitrosylation and yet do not generate NO. We synthesized a series of ethylene glycol–containing molecules (WNNOs; Figure 1), which exhibited different strengths of the N-NO bond, as estimated by Griess assay (diazo-coupling–based dye formation assay under acidic conditions) in 100% H2O (Figure 1).30 The evaluation of water solubility of WNNO6 and WNNO6a-b (see Online Table I) indicated that the triethylene glycol unit affords adequate water solubility. The single carboxylic acid functionality of compound WNNO5 (Figure 1) did not significantly improve the aqueous solubility (2 mmol/L; Online Table I)

  • 2019/02/03 22:40

    The UV-visible spectra were recorded on JASCO V-550 spectrometer. The bandwidth was 2.0 nm, the scan speed was 200 nm/min, and the data acquisition interval was 1 nm. Spectroscopic grade chloroform was purchased and used without further purification. PBS buffer (pH 7.4) was also purchased from Wako Pure Chemicals (Japan). We estimated the kinetic constants of the transnitrosylation reaction of some of the WNNOs with triphenylmethylthiol in chloroform. The second-order rate constants for WNNO in chloroform kobs was obtained under conditions where the concentrations of the N-nitrosamine and the thiol are the same, that is, a single-component approximation of a second-order reaction. That is, to a solution of WNNO (1500 μL, 10 mmol/L) in chloroform (1200 μL), a solution of thiol (300 μL, 50 mmol/L) was mixed at the specified temperature.

  • 2019/02/03 22:39

    All the reagents were commercially available and used without further purification, unless otherwise noted. All the NMR data were recorded on a Bruker Avance 400 NMR spectrometer (400 MHz for 1H-NMR and 100 MHz for 13C-NMR). d-CDCl3 was used as a solvent, unless otherwise noted. Chemical shifts (δ) are reported in ppm with respect to an internal tetramethylsilane (δ=0 ppm) or undeuterated residual solvent (ie, CHCl3 [δ=7.265 ppm]). Coupling constants are given in hertz. High-resolution mass spectrometry was obtained by electron spray ionization time-of-flight detection mode, and the mass spectra were recorded on a Bruker micrOTOF-05. Column chromatography was performed on silica gel (silica gel 60N [100–210 mm]; Kanto Chemicals, Japan).

  • 2019/02/03 22:38

    The biotin switch assay was performed, as described,18 with the following modifications. For in vitro assay, cells overexpressing FLAG-tagged GRK2 (wild type or C340S) were lyzed in lysis buffer (50 mmol/L Tris-HCl [pH 7.4], 1 mmol/L EDTA, 1% Triton X, 200 mmol/L NaCl, and proteinase inhibitors) and centrifuged at 10 000g for 5 minutes at 4°C. The supernatant was immunoprecipitated with monoclonal anti-FLAG antibody by rotating for 2 hours. Protein G was subsequently added and rotated for 1 hour. The resin was washed and resuspended in HEN buffer (250 mmol/L HEPES, pH 7.7, 1 mmol/L EDTA, 0.1 mmol/L neocuproine). Immunoprecipitated FLAG-tagged GRK2 was incubated in an indicated concentration of GSNO, WNNO6, or WNNO7 for indicated times at room temperature in the dark (nitrosylation), subsequently incubated with 20 mmol/L

  • 2019/02/03 22:36

    Phosphorylation of exogenous receptors in HEK293 cells stably expressing FLAG-tagged human β2-AR was quantified by autoradiography. At 1 day before the assay, the cells were seeded into 60-mm dishes. At 20 hours before performing the assay, the cells were pretreated with 100 μmol/L WNNO7 or GSNO, and 4 hours before the assay, the medium was changed into phosphate-free medium supplemented with [32P]orthophosphoric acid (100 μCi/mL) and 1% fetal bovine serum with or without 100 μmol/L WNNO7 or GSNO. At the assay, the cells were stimulated with 10 μmol/L ISO for 10 minutes and put on ice. The cells were then washed twice with ice-cold PBS and lyzed in ice-cold lysis buffer (50 mmol/L Tris-HCl [pH 7.4], 100 mmol/L NaCl, 2 mmol/L Na-orthovanadate, 10 mmol/L Na-PPi, 100 mmol/L NaF, 1 mmol/L dithiothreitol, protease inhibitors, and 1% sodium dodecyl sulfate) on ice by gently shaking each for 5 minutes. After 30 minutes, cell lysates were collected in tube and centrifuged at 20 000 g

  • 2019/02/03 22:35

    The cell surface expression of exogenous receptors in HEK293 cells stably expressing FLAG-tagged human β2-AR was quantified by ELISA.28,29 One day before the assay, cells were seeded into 12-well plates. At 20 hours before the assay, cells were pretreated with 30 μmol/L WNNO7 or GSNO, and 2 hours or 30 minutes before the assay, the cells were pretreated with 10 μmol/L ISO. At the assay, the cells were washed twice with ice-cold 1% BSA-PBS placed on ice for 5 minutes and incubated in an anti-FLAG antibody solution (1:4000) diluted in 1% BSA-PBS for 1 hour at 4°C. This was followed by 2 further washes in 1% BSA-PBS. The cells were next fixed at 4°C for 15 minutes in 4% paraformaldehyde-PBS and again washed twice by 1% BSA-PBS and incubated in anti-mouse horseradish peroxidase–conjugated secondary antibody solution (1:12000) diluted in 1% BSA-PBS for 1 hour at room temperature. This was followed by 2 washes in 1% BSA-PBS for 20 minutes and a final wash in PBS. Finally

  • 2019/02/03 22:33

    cAMP accumulation in intact cells was assayed as described previously.25–27 Briefly, for measuring intracellular cAMP, HEK293 cells or rat cardiac myocytes in 24-well plates prelabeled with [3H]adenine (0.5–2 μCi/well for 24 hours) were washed once with HEPES-buffered DMEM and incubated (37°C for 30 minutes) in the same medium containing 1 mmol/L 3-isobutyl 1-metylxanthine with or without ISO and WNNOs. The reaction was terminated by aspiration and by the immediate addition of ice-cold 5% trichloroacetic acid with 1 mmol/L cAMP/ATP (0.5 mL/well). [3H]cAMP and [3H]ATP were separated on AG 40W-X4 Dowex and alumina columns, and the data are presented as the ratio of [3H]cAMP to [3H]cAMP plus [3H]ATP, as described previously.

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